abstract：:The role of phosphodiesterase in glucagon resistance of large adipocytes was investigated. A comparison was made of phosphodiesterase activities of homogenates prepared from isolated small (mean diameter approximately 45 micro m) and large (mean diameter approximately 78 micro m) adipocytes, using various concentratio...

journal_title：Journal of lipid research

authors： De Santis RA,Gorenstein T,Livingston JN,Lockwood DH

更新日期：1974-01-01 00:00:00

abstract：:Isolated etioplasts from 8-day-old dark-grown pea seedlings incorporated [1-(14)C]acetate into lipid at a relatively low rate. Plastids from seedlings that had been illuminated for at least 2 hr showed an enhanced incorporation provided the plastids were illuminated during incubation with the labeled acetate. Dark inc...

journal_title：Journal of lipid research

authors： Panter RA,Boardman NK

更新日期：1973-11-01 00:00:00

abstract：:A relatively rapid procedure is described for the spectrophotometric determination of total tocopherol in red blood cells (RBC) based on a modification of the original Emmerie-Engel reaction. The critical feature in this method is the presence of a large amount of an added antioxidant, pyrogallol or ascorbic acid, dur...

journal_title：Journal of lipid research

authors： Kayden HJ,Chow CK,Bjornson LK

更新日期：1973-09-01 00:00:00

abstract：:The report that sweat of certain schizophrenics contains the branched chain fatty acid, trans-3-methyl-2-hexenoic acid (TMHA), stimulated an investigation to evaluate the relationship between this fatty acid and schizophrenia. A sensitive and specific gas-liquid chromatography-mass spectroscopic procedure was develope...

journal_title：Journal of lipid research

authors： Gordon SG,Smith K,Rabinowitz JL,Vagelos PR

更新日期：1973-07-01 00:00:00

abstract：:Phospholipase was isolated (in two fractions) from Vipera palestinae venom and it was shown to possess phospholipase A activity (hydrolyzing diacyl-sn-glycerophosphorylcholines, e.g., lecithin, in the 2-position) as well as lysophospholipase (phospholipase B) activity (hydrolyzing 1-monoacyl-sn-glycerophosphorylcholin...

journal_title：Journal of lipid research

authors： Shiloah J,Klibansky C,de Vries A,Berger A

更新日期：1973-05-01 00:00:00

abstract：:Adipose tissue was shown to contain 0.6-1.6 mg of cholesterol per gram wet weight. When expressed per unit of protein or organ mass, fat tissue contains more cholesterol than most other organs or membranes. The cholesterol content of fat tissue increased with the age and weight of the rat. Over 95% of adipose tissue s...

journal_title：Journal of lipid research

authors： Farkas J,Angel A,Avigan MI

更新日期：1973-05-01 00:00:00

abstract：:A synthetic analog of glucocerebroside, N-hexyl-O-glucosyl sphingosine, was found to inhibit the glucosidase in rat spleen that hydrolyzes glucocerebroside. At a concentration of 1 micro m, the analog inhibited the enzyme by 48%. The mode of action appeared to be competitive, probably aided by tight binding of the ami...

journal_title：Journal of lipid research

authors： Erickson JS,Radin NS

更新日期：1973-03-01 00:00:00

abstract：:The mitochondrial lipids from avocado fruit, cauliflower buds, and potato tubers, and the lipids of chloroplasts isolated from avocado fruit and from cauliflower leaves were identified and the concentrations were determined. The lipid composition was compared with that of beef heart mitochondria. Phospholipids constit...

journal_title：Journal of lipid research

authors： Schwertner HA,Biale JB

更新日期：1973-03-01 00:00:00

abstract：:The surface area, A, of contracting fatty acid monolayers was measured as a function of time, t, at constant surface pressure. In the initial temporal phase, ln A was linear with radical t. In a subsequent steady-state phase, ln A was linear with t. The initial desorption coefficient for sodium palmitate, K(i), and th...

journal_title：Journal of lipid research

authors： Patil GS,Matthews RH,Cornwell DG

更新日期：1973-01-01 00:00:00

abstract：:The diurnal cycle of hepatic HMG CoA reductase activity was studied under conditions of controlled feeding where the percentage of dietary lipid, alone or in combination with 2% cholestyramine, was varied. Cholestyramine caused an increase in HMG CoA reductase activity that began soon after feeding started and peaked ...

journal_title：Journal of lipid research

authors： Goldfarb S,Pitot HC

更新日期：1972-11-01 00:00:00

abstract：:Three glycosides of 13-hydroxydocosanoic acid isolated from Candida bogoriensis were characterized by quantitating the amount of carbohydrate, acetate, and hydroxy acid in each, and by gas-liquid chromatography and mass spectrometry of their methyl ester, trimethylsilyl ether derivatives. One of the glycosides was the...

journal_title：Journal of lipid research

authors： Esders TW,Light RJ

更新日期：1972-09-01 00:00:00

abstract：:Quantitative aspects of the binding of free fatty acid to human erythrocytes were studied by measuring the distribution of various amounts of [1-(14)C]lauric acid between washed human erythrocytes and defatted human plasma albumin. Incubations were done at 37 degrees C in an isotonic phosphate-buffered salt solution. ...

journal_title：Journal of lipid research

authors： Spector AA,Ashbrook JD,Santos EC,Fletcher JE

更新日期：1972-07-01 00:00:00

abstract：:Cytolipin R, a ceramide tetrahexoside isolated from rat lymphosarcoma, was studied by sequential hydrolysis with specific glycosidases which revealed the anomeric configurations of the glycosidic bonds. Sugar linkages were established by combined gas-liquid chromatography and mass spectrometry of the partially methyla...

journal_title：Journal of lipid research

authors： Laine R,Sweeley CC,Li YT,Kisic A,Rapport MM

更新日期：1972-07-01 00:00:00

abstract：:Plasma free fatty acid (FFA) transport in human subjects has been studied during the course of prolonged ingestion of different amounts of glucose. Compared with the fasting state, hypocaloric glucose intake resulted in marked suppression of net transport of FFA with no change in (fractional) turnover rate. There was ...

journal_title：Journal of lipid research

authors： Barter PJ,Nestel PJ

更新日期：1972-07-01 00:00:00

abstract：:Methods were developed for the determination of HMG CoA (3-hydroxy-3-methylglutaryl CoA) reductase activity in subcellular fractions of intestinal mucosa and liver of Wistar strain rats. In the liver, reductase activity was located exclusively in the microsomal fraction. In the intestinal mucosa, activity was found in...

journal_title：Journal of lipid research

authors： Shefer S,Hauser S,Lapar V,Mosbach EH

更新日期：1972-05-01 00:00:00

abstract：:An in vitro method is presented which measures valid, unidirectional uptake rates for lipids across the intestinal brush border. This method combines analysis by a newly devised, double isotope counting system for solubilized tissue with the use of a nonabsorbable marker to correct gross uptake determinations for cont...

journal_title：Journal of lipid research

authors： Sallee VL,Wilson FA,Dietschy JM

更新日期：1972-03-01 00:00:00

abstract：:A qualitative iodometric test for lipoxygenase activity is described. The sensitive method is useful for rapidly screening large numbers of samples, as from a chromatography column or after enzyme inactivation treatment in food processing. ...

journal_title：Journal of lipid research

authors： Wallace JM

更新日期：1972-03-01 00:00:00

abstract：:Analyses have been made of glycosphingolipids from visceral organs and brain of a patient with an unusual lipid storage disorder diagnosed initially as classical Tay-Sachs disease. Levels of the lipids from fresh-frozen sections of gray and white matter, kidney, spleen, liver, and heart from this patient were compared...

journal_title：Journal of lipid research

authors： Snyder PD Jr,Krivit W,Sweeley CC

更新日期：1972-01-01 00:00:00

abstract：:When NH(4)OH-NH(4)Cl extracts of adipose acetone powder were applied to agarose gel chromatography columns, two peaks of lipoprotein lipase were eluted. The first activity peak (LPL(a)) was eluted with an elution volume of a protein of molecular weight approximately five times that of the second (LPL(b)). Addition of ...

journal_title：Journal of lipid research

authors： Garfinkel AS,Schotz MC

更新日期：1972-01-01 00:00:00

abstract：:In isolated fat cells, the same maximal rate of glycerol production can be induced by epinephrine or ACTH, alone or in combination with each other or with glucagon. With fat cells from rats weighing 150-175 g, the maximal rate of lipolysis attained with glucagon was 75-80% of that produced by epinephrine or ACTH, and ...

journal_title：Journal of lipid research

authors： Manganiello V,Vaughan M

更新日期：1972-01-01 00:00:00

abstract：:The following data have been obtained from comparative studies on the metabolism of cholesterol, cholestanol, and beta-sitosterol by L-cell mouse fibroblasts. (1) When the sterols are added to the growth medium under similar conditions, cellular incorporation of cholesterol > cholestanol > beta-sitosterol; (2) only li...

journal_title：Journal of lipid research

authors： Rothblat GH,Burns CH

更新日期：1971-11-01 00:00:00

abstract：:Liver microsomal lipid peroxidation has been observed in fatal human CCl(4) poisoning, in rats with fatty livers induced by CCl(4) or by yellow phosphorus, and in mice poisoned with 1,1,2,2-tetrachloroethane. These observations suggest the possibility that other instances of toxic liver injury may involve lipid peroxi...

journal_title：Journal of lipid research

authors： Srinivasan S,Recknagel RO

更新日期：1971-11-01 00:00:00

abstract：:The chromatographic behavior of 1,2-, 1,3-, 1,4-, and 1,12-long-chain alkane diols and 1-O-alkylglycerols and their derivatives has been compared. Thin-layer chromatography on Silica Gel G gives poor separations of the 1,2-, 1,3-, and 1,4-alkane diols, O-alkylglycerols, and some of their isopropylidene derivatives. Ho...

journal_title：Journal of lipid research

authors： Blank ML,Cress EA,Stephens N,Snyder F

更新日期：1971-09-01 00:00:00

abstract：:14C-labeled oleic acid and (3)H-labeled monoether in a bile salt solution were perfused through three types of plastic tubing. Large proportions of lipid were lost to the walls of silicone rubber and polyvinyl chloride tubes. The major portion of the lipid lost was recoverable only when chloroform-methanol was perfuse...

journal_title：Journal of lipid research

authors： Lee KY

更新日期：1971-09-01 00:00:00

abstract：:The enzymic incorporation of choline-1,2-(14)C from CDP-choline-1,2-(14)C into phosphatidylcholine by spinach leaf preparations was characterized. The enzyme catalyzing the incorporation, choline phosphotransferase, had a pH optimum of about 8.0 and required either Mn(2+) or Mg(2+) as cofactor. The saturation concentr...

journal_title：Journal of lipid research

authors： Devor KA,Mudd JB

更新日期：1971-07-01 00:00:00

abstract：:Lipoprotein electrophoresis on agarose gel has been modified to allow estimation of the absolute quantity of each fraction. The reproducibility of the method is illustrated by 12 determinations in a single day on serum from one normal subject: mean total dye uptake was 302 +/- 9 (sd "corrected dye units," and the perc...

journal_title：Journal of lipid research

authors： Hulley SB,Cook SG,Wilson WS,Nichaman MZ,Hatch FT,Lindgren FT

更新日期：1971-07-01 00:00:00

abstract：:Lipid-containing deposits within the swim bladders of Coryphaenoides acrolepis and Antimora rostrata were investigated. Lipid analysis of this material, which was quite uniform from the two species, yielded the following data: neutral lipids, 36.0-41.7%; phospholipids, 53.6-56.7%; and glycolipids, 4.3-8.9%. Cholestero...

journal_title：Journal of lipid research

authors： Patton S,Thomas AJ

更新日期：1971-05-01 00:00:00

abstract：:The effect of various levels of the oral contraceptive drug, Enovid E, on serum and liver lipid levels of adult female rats has been investigated. Doses ranging from 0.052 to 1.04 mg/day have been employed in rats fed control or cholesterol-containing diets. It has been confirmed that after administration of even low,...

journal_title：Journal of lipid research

authors： Aftergood L,Alfin-Slater RB

更新日期：1971-05-01 00:00:00

abstract：:Rat serum lipoproteins were separated into at least four fractions by agarose-starch gel electrophoresis. The system used was discontinuous in that glycine and sodium barbitone buffer was used in the reservoirs and Tris buffer was used for the gels. The four major bands could be related to the pattern obtained by ultr...

journal_title：Journal of lipid research

authors： Chalvardjian A

更新日期：1971-05-01 00:00:00

abstract：:The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a magnesia-silica gel ...

journal_title：Journal of lipid research

authors： Saito T,Hakomori SI

更新日期：1971-03-01 00:00:00

abstract：:In a previously described method for determining the activity of cerebroside galactosidase, the enzyme preparation was incubated with an emulsion of cerebroside which had been labeled in the galactose moiety. The liberated galactose was separated from the emulsion by liquid-liquid partitioning, but the presence of det...

journal_title：Journal of lipid research

authors： Radin NS,Arora RC

更新日期：1971-03-01 00:00:00

abstract：:Biosynthetic pathways of phosphatidylcholine and triglyceride were studied in proliferating hepatic endoplasmic reticulum of rats pretreated with phenobarbital. Phosphatidylcholine accounted for the major increment in membrane phospholipid. In vitro measurements of hepatic microsomal enzymes which catalyze phosphatidy...

journal_title：Journal of lipid research

authors： Young DL,Powell G,McMillan WO

更新日期：1971-01-01 00:00:00

abstract：:The lipids of intact human platelets were labeled in vitro with acetate-1-(14)C, and the distribution of radioactivity in individual fatty acids and in lipid classes was examined in platelet subcellular fractions separated by sucrose density gradient ultracentrifugation. The distribution of newly formed fatty acids am...

journal_title：Journal of lipid research

authors： Deykin D

更新日期：1971-01-01 00:00:00

abstract：:Phosphorylcholine-1,2-(14)C and choline-1,2-(14)C-labeled cytidine diphosphate choline are incorporated into lecithin by whole homogenates and particulate fractions of rat retina with optimal incorporation of label by the microsomal fraction. The soluble fraction contains a factor(s) which stimulates incorporation of ...

journal_title：Journal of lipid research

authors： Swartz JG,Mitchell JE

更新日期：1970-11-01 00:00:00

abstract：:Albumin-bound long-chain fatty acid methyl esters (ME) were taken up and utilized by Ehrlich ascites tumor cells and slices of rat heart, liver, and kidney. Much more ME than albumin was taken up by the tumor cells, indicating that ME dissociated from the carrier protein during their uptake. 70-80% of the radioactivit...

journal_title：Journal of lipid research

authors： Kuhl WE,Spector AA

更新日期：1970-09-01 00:00:00

abstract：:A method for the preparation of 1-alkyl 2-acyl glycero-3-phosphorylcholine, labeled in the 2-position with oleic acid-1-(14)C is described. This method, based on the acylation of a 1-alkyl glycero-3-phosphorylcholine-CdCl(2) complex which is, in turn, formed from 1-alk-1-enyl glycero-3-phosphorylcholine by hydrogenati...

journal_title：Journal of lipid research

authors： Poulos A

更新日期：1970-09-01 00:00:00

abstract：:The total nitrogen and L-hydroxyproline concentrations of two intra-abdominal and two subcutaneous sites of adipose tissue were measured in male and female Wistar rats. At 100 g body weight the nitrogen concentration of intra-abdominal adipose tissue was slightly lower than that of subcutaneous adipose tissue. As age ...

journal_title：Journal of lipid research

authors： Curtis-Prior PB,Hanley T,Trethewey J,Stewart GA

更新日期：1970-07-01 00:00:00

abstract：:The effect of microbiological deconjugation of bile acids on total bile acid and neutral sterol fecal excretion by adult male rats has been studied. A screening method utilizing mice allowed selection of a Clostridium perfringens type A strain, which accelerated cholesterol catabolism in mice. When this species of bac...

journal_title：Journal of lipid research

authors： Kellogg TF,Knight PL,Wostmann BS

更新日期：1970-07-01 00:00:00

abstract：:Ceramides containing phytosphingosine as base and one of the fatty acids 16:0, 18:0, 20:0, 22:0, 23:0, and 24:0, were prepared by direct coupling in the presence of a mixed carbodiimide. The ceramides were analyzed as the 1,3,4-tri-O-trimethylsilyl ether derivatives by gas-liquid chromatography-mass spectrometry. Gas ...

journal_title：Journal of lipid research

authors： Hammarström S

更新日期：1970-05-01 00:00:00

abstract：:Glycolipids were isolated from the brain of a patient with a myoclonic variant of late infantile amaurotic idiocy. There was an abnormal glycolipid pattern in gray and white matter. The observed high concentration of gangliosides was due to a uniform accumulation of all four major gangliosides and was not limited to o...

journal_title：Journal of lipid research

authors： Bartsch GG

更新日期：1970-05-01 00:00:00